3. SoftMax® Pro Software for Windows 10 and 11 is designed to provide the simplicity, flexibility and power required for advanced data analysis. Detailed specifications of each model reader are reported in Section 7, “Specifications”. 7 cells were co-transfected with 0. When the plate was read on the SpectraMax M5 microplate reader, an enzyme concentration range of 0. Figure 4: Transcreener ADP. played in a grid format that corresponds to the wells in a microplate (all instruments) or individual cuvettes (using SpectraMax Plus, Plus384, M2, M2e, M5e or M5). Biotek FLx800 Fluorescence Microplate Reader; Related products-20%. With optimized reagents and the industry leading data acquisition and analysis tool, SoftMax® Pro 7 Software, the SpectraMax to perfectly match your needs. The spectrophotometric. Launch SoftMax® Pro Software and open the PicoGreen fluorescence protocol from the Protocols dropdown menu. As readout, fluorescence was measured at Ex/Em = 540/590 nm using a SpectraMax M5 microplate reader with SoftMax Pro v7 (Molecular devices) after 4–6 h of incubation in normal cell-culture medium. Page 27 SpectraMax M2 and M2e Microplate Reader User Guide Place a standard 1 cm cuvette that contains the aqueous/solvent mixture you use for the plate samples into the cuvette port. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance in one multimode reader package. (Shanghai, China). Molecular Devices Corporation. 0. This document is copyright protected and any reproduction of this document, in whole or any part, is strictly prohibited, except as Molecular Devices may authorize in. The reader can be equipped with a NanoBRET detection for a 4-fold improvement in limit of. SpectraMax® Aquamax. Table 3 shows the ratios obtained on all Molecular Devices plate readers tested for the minimum required assay control dilutions for each assay. Microplate readers from Molecular Devices are designed to provide consistent performance for many years. SpectraMax iD5; SpectraMax Mini; SpectraMax Mini Install Guide; SpectraMax Mini Reader Plug-In Download Page (required for SoftMax Pro 7. Five preferred modes for diverse bioresearch applications. 1. Do not touch the clear surfaces of the cuvette. Introduction. S. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. SpectraMax L Microplate Reader is a sensitive luminometer with programmable injector options that measures flash and glow luminescence assays, including dual luciferase reporter gene, G protein-coupled receptor (GPCR) in 96- and 384-well plates. SpectraMax® Plus 384 Absorbance Plate Reader, Molecular Devices. SoftMax Pro. Microplate Reader, MultimodeProducts : SpectraMax M2/M2e reader, SpectraMax Plus 384 reader, SpectraMax M4 reader, SpectraMax M5/M5e reader, SpectraMax M3 reader. Compatible automation solutions for SpectraMax Readers. Examples: “Press carefully to engage,” “ Do not. Are you using the correct Microplate Adapters? For the SpectraMax M3/M4/M5 readers - use the Purple Assay Plate adapter for Top Read only. Multi-Mode Microplate Readers including the M3, M4, M5, and M5. Figure 1. FlexStation® 3 Multi-Mode Microplate Reader. Qualify the fluorescence performance of the SpectraMax . The SpectraMax® M3, M4, M5 and M5e readers offer a modular, upgradeable dual monochromator microplate reader platform with a wide range of high performance multi. From the Windows Start menu, go to All programs > Molecular Devices > SoftMax Pro x. Figure 1. Western blot analyses. We use Spectramax M5 microplate reader in our lab. Other readers with cell imaging include PerkinElmer’s EnSight™ Multimode Plate Reader and Molecular Devices’ SpectraMax i3x Multi-Mode Microplate Reader. The SpectraMax M5 features a triple-mode cuvette port and a single-read command capability for experiments requiring multiple detection parameters. This is equivalent to having 511 built-in filters,The SpectraMax M5 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-, and 384-well microplate. The LMax plate reader has similar calculations and its RLUs are comparable to those of LMax II 384. SPECTRAmax PLUS 384 Microplate Spectrophotometer Operator’s Manual vii Conventions Used in this Manual The names of keys that appear on the SPECTRAmax PLUS 384 control panel are shown in boxed Helvetica type. TR-FRET assay. . The following document is intended to demonstrate setup of this instrument. The integration time suggested is 5-10 sec. IMAP assays are based on binding of. Step 2. 7°C (gradient heating design). Molecular Devices Spectramax M5 Microplate Reader. The raw data from each individual experiment were normalized to vehicle-treated HK-2. For example,it is already turned on and the screen should look like this with “SpectraMax M5” showing as connected in the top left: 7. The system also gives users the flexibility to choose wavelengths between 200 nm and 1000 nm. For more detailed information and technical support of Life Technologies assays, please call 1-800-955- 6288 and enter extension 40266 or email drugdiscoverytech@lifetech. : 915 515 403 • Fax: 914 334 545 • e-mail: info@bionova. Figure 4: Transcreener ADP. The temperature of the microplate chamber can be regulated, if desired from 4°C above ambient to 45°C. Path Check Pathlength Measurement SpectraMax Spectrofluorometers. To quantify the A1AT in rat serum, standard curves were drawn for nA1AT, rA1AT-WT and rA1AT mutants, respectively. SpectraMax® Microplate Readers and SoftMax® Pro Software. For more detailed information and technical support of Life Technologies assays, please call 1-800-955- 6288 and enter extension 40266 or email [email protected]% Triton X‐100, 10 mM potassium phosphate buffer pH 7. Choose from a three- (M3), four- (M4), orWe would like to show you a description here but the site won’t allow us. The Molecular Devices SpectraMax® M5/M5e Microplate Reader was tested for compatibility with Life Technologies Omnia® Assays. Maximum signal with lowest background. SpectraMax® M5e. The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. Before you Begin Materials Required but Not Provided • Fresh, anhydrous. Spectramax M5 microplate reader: Molecular Devices: N/A:. The SpectraMax® ABS Plus Microplate Reader can run . Urea-induced unfolding of rA1AT-WT and the Q9N mutant was measured using a SpectraMax. Self Service Available. Read one sample or up to 384 inThe SpectraMax® M2 and M2e readers provide two detection modes in one platform, and offer the the added functionality of bottom-read fluorescence for cell-based assays (M2e). After being incubated in the dark at 60 ℃ overnight, the supernatants were centrifuged and optical densities were measured at 750, 665, 652 and 470 nm using the SpectraMax M5 microplate reader (Molecular Devices,. TNF standard curve. TR-FRET assay principle. Fluorescence Microplate Reader Comparison Chart. 2. E Models. When you flip the LM1 plate over for checking Bottom Read, remove the Purple Plate adapter. on the SpectraMax Multi-Mode Microplate Readers by issuing a single. Molecular Devices SpectraMax® series microplate readers. 2. " The SpectraMax M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. Go to Product Details. A microplate reader that can detect two or more applications is considered a multi-mode plate reader. Introduction. The dual monochromator optics allow the widest. 1. The SpectraMax® M Series Multi-Mode Microplate Readers are modular and upgradeable with a wide range of high performance capabilities ideal for life science research and. SpectraMax M5 reader settings are shown. edu) to be added as a trained user in iLab. l. com. Western blotting analysis. l. FlexStation™ II. ) by nonlinear regression using. on the SpectraMax Multi-Mode Microplate Readers by issuing a single. For biological applications involving small samples, such as purificationThe guidelines for cuvette use in SpectraMax instruments are the same as those that apply to any high-quality spectrophotometer. The fluorometric performance of the SpectraMax M2 e is similar, but slightly superior to that of the Gemini EM, a dedicated top and bottom read fluorescence microplate reader. Then, 150 μL of dimethyl sulfoxide was added to each well and shaken gently for 10 min. The SpectraMax ® i3x Multi-Mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with the added functionality of. M3-M5e_manual. When the plate was read on the SpectraMax M5 microplate reader, an enzyme concentration range of 0. SpectraMax M5 microplate reader (Molecular Devices, California, USA) with excitation at 450 nm and emission at 570 nm. Choose from a three- (M3), four- (M4), oron the SpectraMax Multi-Mode Microplate Readers by issuing a single. 6. The trademarks used herein are the property of Molecular Devices, LLC or their respective owners. 05 3. Wide range of applications The VersaMax reader covers. SpectraMax iD3, iD5, i3x, i3, M3, M4, M5, M5e, SpectraMax L, 6186 Luminescence FlexStation 3. for the SpectraMax M3/M4 and FlexStation® 3 Multi-Mode Microplate Readers. The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. FlexStation™ I. Preparation of the peritoneal macrophages The peritoneal macrophages were obtained from the mice after intraperitoneally (i. Standard 6-, 12-, 24-, 48-, 96- and 384-well microplates. SpectraMax iD5 Multi-Mode Microplate Reader, a hybrid monochromator- and filter-based, five-mode plate reader with automatic filter identification and western blot capability. Using an SpectraMax Microplate Reader for DNA absorbance test, there is one problem that the A260/A280 is always wrong, sometimes half plate wrong and sometime, several lanes. The SpectraMax ® M3, M4, M5, and M5 e Microplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a. The SpectraMax® absorbance spectrophotometers and plate readers provide the versatility and convenience for a wide range of assays such as ELISAs, nucleic acid and protein quantitation, and microbial growth. book Page 7 Wednesday, July 7, 2010 2:09 PM. 1** Version 7. SpectraMax Lmicroplate luminometer. It provides ready-to-run protocols, analysis algorithms, and 21 different curve fit options. SpectraMax i3/i3x and Paradigm readers can be equipped with the HTRF detection cartridge. Fill a clean plate with water. Description 2 SpectraMax M2 & SpectraMax M2e Multi-mode Plate Readers Operator’s Manual. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. 7 cells were pretreated with SG (0, 25, 50, and 100 μg/mL). The reader uses two scanning monochromators to determine the optimal excitation and emission settings for the spectral characteristics of. The SpectraMax ® M3, M4, M5, and M5 e Microplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a. The data acquisition and analysis is easily streamlined when using the preconfigured protocol in Softmax Pro Sofware. on Molecular Devices microplate readers: SpectraMax® iD5, i3x, Paradigm®, and M5 Multi-Mode Microplate Readers. AlphaScreen and AlphaLisa detection using a 680 nm excitation laser diode and a 570/100 nm emission filter. M5 SpectraMax M5e Absorbance X X X X Fluorescence intensity X X X X Fluorescence polarization XX Time-resolved fluorescence XX X Luminescence x x X X Note: In this user guide, all references to SpectraMax Multi-Mode Microplate Readers include the M3, M4, M5, and M5e models. 5 Compatible microplate readers FP Plate Reader SpectraMax® M5/M5e Multi-Mode Microplate ReaderSpectraMax M5 Multi-Mode Microplate Reader; Method. B: Selection of Excitation Wavelength. Products : SpectraMax i3x reader, SpectraMax iD5 reader, SpectraMax iD3 reader, General, FilterMax F5 reader, SpectraMax ABS reader, SpectraMax L reader, SpectraMax M5/M5e reader This page contains the table of contents of resources available for the SpectraMax Microplate Reader Suite of Products. Figure 1. Validating the absorbance optical performance of your microplate reader is as simple as reading a plate. Pick the best speed, sensitivity, and price for your needs. SoftMax Pro Software can collect data from one or more microplates or cuvettes and store it in a single data file, using the same or different instrument settings for different microplates or cuvettes. 3 Version 7. This application note describes the use of the SPECTRAmax ® GEMINI XS from Molecular Devices to study the performance of the LIVE/DEAD ® Viability/Cytoxicity Assay Kit for animal cells. The ultimate guide to microplate readers is an eBook that tells you everything you need to know about plate reader solutions from what a microplate reader is, to popular applications and technology, to GxP compliance and special tips and tricks to help maximize your. The data acquisition and analysis is easily streamlined when using the preconfigured protocol in Softmax Pro Sofware. iD3, iD5, i3, i3x, M3, M4, M5, M5e, L, and FlexStation 3 readers. 67. Microplate Data Acquisition and Analysis Software, users are now able to extend the ease-of-use of their typical plate reader applications to cell-based imaging and western blot detection. In the M2, M5 and Plus. The SpectraMax ® i3x Platform from Molecular Devices is a multi-mode microplate reader that evolves with your future needs and offers an unlimited breadth of application possibilities. The dual monochromator optics allow the widest range of applications to be utilized for bioresearch and drug discovery applications, all without the need to change. When a SOP Title: Use and Maintenance of a Molecular Devices M5 Plate Reader Document ID: HSL_EQ_005 Version 2. Cell viability was assayed by addition of BacTiter-Glo ™ bioluminescence reagent according to the manufacturer’s protocol (Promega) and luminescence measured using a Spectramax M5 microplate reader (Molecular Devices). System Diagrams and Dimensions. Note: In this user guide, SpectraMax ® refers to several SpectraMax . Italic and boldface type are used for emphasis. Molecular Devices SpectraMax M5 ; Molecular Devices SpectraMax M2. We would like to show you a description here but the site won’t allow us. 1. With optimized reagents, option to operate the. Efects of luciferase concentration, luciferin concentration, ATP concentration, pH, temperature, and reaction time on the. Microplate Readers include the M3, M4, M5, and M5e models. Cuvette port functionality can also be controlled using SoftMax Pro Software. The AlphaScreen Detection Cartridges utilize a 680nm Laser Diode to provide a sensitive reading system for AlphaScreen assays. Description: The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research. Microplate Readers include the M3, M4,. Compact and tunable absorbance microplate readers DATA SHEET. ,. In SOFTmax PRO, set up a plate section for an excitation scan with no cutoff filter and use the medium PMT setting. FilterMax F3 and F5 Multi-Mode; SpectraMax Paradigm® Multi-Mode. Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI). Catalog Number: (89212-396) Supplier: Molecular Devices. Add to Cart. Note: this assay can also be detected using the luminescence mode of SpectraMax M5/M5 e and FlexStation 3multi-mode microplate readers. 04 to 3. The SpectraMax® ABS and ABS Plus absorbance microplate readers provide the flexibility, sensitivity, and convenience for a wide range of assays such as ELISAs, microbial growth, and protein quantitation. LMax II 384 Microplate Luminometer: Integration time = 1 second, Settling time = off, Autoread = off. Click SoftMax Pro Software User Guide. Molecular Devices Spectramax M5 Microplate Reader; Related products. NOTE: Limited support and literature is available for the SpectraMax 250 due to the senescence of the product. Learn how you can utilize microplate readers to drive landmark discoveries. CAT activity was measured using SpectraMax M5/M5 Microplate Reader (Molecular Devices, MDS Analytical Technologies). Molecular Devices. It cannot be used for luminescence measurements. 87. 305 reference level saturation. 0112-0115G October2018 SpectraMax®M3 SpectraMax®M4 SpectraMax®M5 SpectraMax®M5e Multi-ModeMicroplateReaders UserGuideSpectraMax® iD3, iD5, ABS, and ABS Plus readers, and up to 2. 0 out of 5 $ 24,695. Molecular Devices SpectraMax ® i3x MultiMode Microplate Reader, in combination with luminescent assays for cell viability and cytotoxicity, provides a sensitive and rapid way to measure the number of viable cells in culture and quantify the cytotoxic effects of experimental treatment. The acceptance. IMAP FP Settings in SoftMax Pro M5 SpectraMax M5 e Absorbance X X X X Fluorescence intensity X X X X Fluorescence polarization XX Time-resolved fluorescence XX X Luminescence x x X X Note: In this user guide, all references to SpectraMax Multi-Mode Microplate Readers include the M3, M4, M5, and M5 e models. If you do not have an iLab account you must first make one: Molecular Devices SpectraMax M3 Multi-Mode Microplate Reader is a dual-monochromator, multi-detection microplate reader with full spectral range detection for cuvettes and 6-, 12-, 24-, 48-, 96-, and 384-well microplates. VersaMax™ Microplate Reader (Serial numbers begining with BNxxxxx and BNRxxxxx are supported) SpectraMax® Plus 384 Absorbance Microplate Reader; SpectraMax® L (software version 5. Both readers feature an 8-channel read head for fast reads and. This user guide provides detailed instructions, troubleshooting tips and technical specifications for these instruments. E Models Only) 69. The dual monochromator optics allow the widest range of applications to be. Page 28 Chapter 5: Read Modes and Read Types 1. Between steps, reactions were rinsed 3 times with TBS + 0. "With the launch of the Molecular Devices® SpectraMax® M3 and M4 Multi-Mode Microplate Readers, we now offer a full range of multi-mode detection capabilities within our SpectraMax® product line," said Andy Boorn, President of MDS Analytical Technologies. Bio-Tek BioTek uQuant Microplate Reader Complete w WARRANTY $3,450. The SpectraMax® M5e Multi-Mode Microplate Reader delivers single-mode reader performance in one multi-mode reader package. Optimal excitation APPLICATION NOTE and emission wavelengths for SpectraMax M5 fluorescence microplate readers. Label-free detection. The introduction of the Molecular Devices’ SPECTRAmax PLUS microplate spectrophotometer marks the first time that photometric measurements made in a microplate format can be automatically normalized such that the resultant values are identical to those obtained with the corresponding solutions in a standard 1 cm. analysis, controls the SpectraMax Multi-Mode Microplate Readers. spr. 2. degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader systems with all leading partner. . The dual monochromator optics allow the widest range of applications to be utilized for bioresearch and drug discovery applications, all without the need to change. Fluorescence Microplate Readers. or multimode readers such as the SpectraMax M5, Analyst HT or Analyst GT. The assay window spanned four orders of magnitude. Bottom-reading is usually recommended for cell-based assay with adherent cells. 00 USD. The dried paper microwell plate was then placed in the SpectraMax M-5 microplate reader, and the signal intensities were measured for each well. For 40 years, we have partnered with scientists to help them achieve landmark discoveries. TR-FRET assay. SpectraMax® Aquamax. Table of Contents. The chamber contains springs that position the cuvette in the proper alignment for a read. Description: This UV-visible absorbance microplate reader provides ultrafast, full spectral range detection for cuvettes, 96-well, and 384-well microplates, with t. User guide for SpectraMax M5 Microplate Reader Before beginning, you should contact Tatiana Laremore (tnl1@psu. Five preferred detection modes. The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. Fluorescent signals were measured using the SpectraMax/M5 fluorescence spectrophotometer plate reader (Molecular Devices Co. View the Molecular Devices website. Three independent experiments were performed. The SpectraMax® iD3 Multi Mode Microplate . Recertification is recommended every two years. Turn on the microplate reader. FlexStation™ I. The 1/absorptivity value for double stranded DNA at 260 nm is commonly assumed to be \~50 µg/mL for a 1-cm pathlength3,4. According to Biocompare, “one of the most overlooked pieces of equipment in any lab is the microplate reader. SpectraMax Microplate Readers. 6-384 (96- and 384-well with pipetting) 6-384. temperature and fluorescence was measured at Ex/Em 490/585 nm using a Molecular Devices SpectraMax M5 microplate reader. The SpectraMax M5 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-, and 384-well microplates. anisotropy was measured on SpectraMax M5 microplate reader with 485 nm excitation and 535 nm emission after 60 min incubation at room temperature. 67. Data are normalized to vehicle-treated. RAW 264. Absorbance Detection Cartridges combine scanning and broad spectrum wavelength range to address a variety of nucleic acids, proteins, ELISAs and immunoassays found in the laboratory. The SpectraMax® L Microplate Reader measures flash and glow luminescence assays in both 96- and 384-well plates. How do I set up Hellma Cuvette validation set recertification? Send Hellma cuvette standards to Hellma. The Molecular Devices SpectraMax 384 Plus is a UV/Vis spectrophotometer and microplate reader combination for more reliable and fast data reporting with built-in cuvette port and microplate drawer. However, this method is only able to measure down to about 250 ng/mL on a typical absorbance microplate reader. Our multi-mode plate readers provide great flexibility and include absorbance, fluorescence and luminescence with configurable options for fluorescence polarization (FP), time. ppr - M3, M4, M5, M5 SpectraMax readers (in SMP 4. Comprehensive software and validation Comprehensive data analysis and GxP solutions SoftMax. 0 out of 5 $ 17,999 $ 16,995. 8 µg pNF-κB-After 3 h of incubation at 37 °C, the medium was gently removed, and a 150-μL aliquot of DMSO was added to each well. 5. The readings with excitation at 540 nm and emission at 600 nm represented the amount of phosphorylated IκB-α in the cells. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. The Molecular Devices SpectraMax M3 Multi-Mode Microplate Reader is a dual-monochromator, multi-detection microplate reader with full spectral range detection for cuvettes and 6-, 12-, 24-, 48-, 96-, and 384-well. 40266 Email: drugdiscoverytech@lifetech. The SpectraMax M5 features a triple-mode cuvette port and a single-read command capability for experiments requiring multiple detection parameters. 1 Version 7. A serial dilution of IgG standards was performed, using XP Media/L-glutamine as the diluent, to concentrations from 2. 00. The detection of lactate dehydrogenase releaseBriefly, algae were harvested, centrifuged, and re-suspended with an equal volume of methanol at the end of the experiment. However, when a specific probe is not available, or it causes off-target effects on the. free tracer decreases, resulting in an. A microplate reader asThe SpectraMax® Quant™ dsDNA Assay Kits are designed for fluorescence-based dsDNA quantitation across a broad range of concentrations. The user must ensure that the meniscus is comfortably above the light beam in standard cuvettes and that the sample chamber in a microcuvette is aligned properly with the beam. Spectrofluorometers or plate readers with fluorescence detection typically offer greater sensitivity and a wider dynamic range compared to absorbance detection. Thank you!. 0 μL) instead of the enzyme solution was used as the sample blank group. Next, add 10 μl of the MTT labeling reagent (5 mg/mL) to each well for 4 h. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. The SpectraMax® M5 Multi-Mode Microplate Reader is ideally suited for assay development and high-throughput screening using IMAP assays. SpectraMax M3, M4, M5, and M5e Microplate Reader User Guide Cuvette Chamber The cuvette chamber is located to the right of the control panel. Plasma Tau was determined by high-performance liquid chromatography (Shimadzu ®, model LC 10AD) using a Shimadzu model RF 535 fluorescence detector. The plate is then covered and mixed to allow complete cell lysis before the luminescent signals are read on the SpectraMax i3x Multimode Microplate Reader (Figure 1). Microplate Readers include the M3, M4, M5, and M5e models. book Page 1 Wednesday, July 7, 2010 2:09 PMThe SpectraMax M2/M2e User Guide is a comprehensive manual that provides detailed instructions on how to operate, maintain, and troubleshoot the SpectraMax M2 and M2e microplate readers. Here is the problem: Using an SpectraMax Microplate Reader for DNA absorbance test, there is one problem that the A260/A280 is always wrong, sometimes half plate wrong and sometime, several lanes. The SpectraMax M5 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-, and 384. 313 reference gain check fail. Cavicide (Warehouse, Cat # 79300360). A: Microplate Preparation. 2 Figure 3 shows the same assay plate read on the Analyst HT multi-mode reader. 306 plate air cal. TNF standard curve. both cuvette-based and microplate reader applications on the same instrument. Flexible for 6-well to 384-well microplates and cuvettes. flexibility of monochromator-based systems. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. Fluorescence intensity was detected by. Molecular Devices recommends that you use high-quality, doubleshielded cables to connect your SpectraMax. Fill a clean plate with water. upgradeable with a wide range of high performance capabilities ideal for life science research and drug. SpectraMax M Series Multi-Mode Microplate Readers 7 The SpectraDrop Micro-Volume Microplate offers the ability to use as little as 2µL samples with 24- or 64-well plates. A preconfigured protocol with optimized reader settings enabled reading of a 384-well plate in just two minutes. 72 MB. Ferguson, Ph. The Molecular Devices SpectraMax® M5/M5e Microplate Reader was tested for compatibility with Life Technologies GeneBLAzer® assays. The SpectraMax M5e Multi-Mode Microplate Reader features a triple-mode…. When a The SpectraMax M5 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-, and 384-well microplates. Choose from a three- (M3), four-. Introduction. Table of Contents. IMAP FP PDE Evaluation Kit (Molecular Devices P/N R8175)The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. . The SpectraMax M5 features a triple-mode cuvette port and a single-read command capability for experiments requiring multiple detection parameters. The absorbance values at 450 and 570 nm were determined using the SpectraMax M5 microplate reader (Molecular Devices, Silicon Valley, CA, United States). Maximum signal with lowest background. SpectraMax® M5e Multi-Mode Microplate Reader performance with an integrated 8- or 16-channel pipettor into one compact benchtop reader. 40266 Email: drugdiscoverytech@lifetech. or multimode readers such as the SpectraMax M5, Analyst HT or Analyst GT. After 24 h drug treatment, cells were lysed by RIPA buffer (Life Technologies Inc, US). FlexStation™ III. Materials. Molecular Devices Spectramax M5e Multi Mode Microplate Reader; Related products. Incubation to 70 o C enables high temperature assays such as enzyme kinetics, protein stability/aggregation. The bound bio-peptidomimetics were recognized by Eu 3+-SA (diluted using 1% BSA in TBS), and the fluorescence intensities were measured using a SpectraMax M5 microplate reader after adding 200 μL signal enhancement solution, which the parameters were set as the followed: excitation and emission wavelengths of 340 and 615 nm,. SoftMax® Pro Microplate Data Acquisition and Analysis Software Version 6 User Guide 5014177 C October 2011 FrameMaker_Export. Validate the optical performance of your absorbance . Molecular Devices SpectraMax® series microplate readers. Insert the filled microplate into the drawer, matching well A1 with position A1 in the drawer. SpectraMax M3 M4 M5 M5e SpectraTest FL1 revA. Examples: “Press carefully to engage,” “ Do not. Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI) and have optical performance comparable to a top-of-the-line dedicated spectrofluorometer or. Fluorescence was measured (Ex 530/Em 590) in a SpectraMax M5 microplate reader. SpectraMax M3, M4, M5, and M5 e Multi-Mode Microplate Readers 0112-0115 F 9 PathCheck ® Pathlength Meas urement Technology A SpectraMax Multi-Mode Microplate Reader with PathCheck Pathlength Measurement Technology allows normalization of variable well volumes to 1-cm cuvette readings. Choose cables that meet the requirement described in this appendix. Qualify the luminescence performance of the SpectraMax . ATP standard curves read on SpectraMax microplate readers. The ultimate guide to microplate readers is an eBook that tells you everything you need to know about plate reader solutions from what a microplate reader is, to popular applications and technology, to GxP compliance and special tips and tricks to help maximize your. The Molecular Devices SpectraMax 384 Plus is a UV/Vis spectrophotometer and microplate reader combination for more reliable and fast data reporting with built-in cuvette port and microplate drawer. Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. The SpectraMax 190 reader can be integrated with Molecular . After 3 min of reaction, the fluorescence emission spectra of the mixed solutions were recorded using a multifunctional microplate reader (SpectraMax M5, Molecular Devices, USA) at the excitation wavelength (λ ex) of 485 nm. We would like to show you a description here but the site won’t allow us. April 22 Asking Price: $15,990 USD Shoukat Mumtaz / Zantek Medical phone: +1 (952) 657-7977 (3). 2. The following document is intended to demonstrate setup of this instrument. SpectraMax iD5 Multi-Mode Microplate Reader. This monochromator-based instrument allows users to select optimal wavelengths for any chosen fluorophore without the need to purchase additional filter sets. Learn how to set up protocols, customize reports, export results, and more with this PDF document. fluorescence polarization, trf, fret, igg quantitation, elisa, western blot, spectramax i3x, spectramax id5 multi-mode microplate reader, spectramax id5, spectramax paradigm, spectramax m5, spectramax m5 multi-mode microplate reader, high-throughput igg quantitation, mab igg titer, softmax pro, valita titer, valitatiter. Plates are used to validate optical performance of SpectraMax M3, M4, and M5/M5e readers. Our absorbance plate readers feature our PathCheck Sensor. Turn on the microplate reader. The BioTek Synergy H1 multimode microplate reader is modular and upgradable to include variable-bandwidth monochromator optics for fluorescence excitation and emission wavelength selection, plus luminescence and UV-Vis absorbance. Wide range of applications. Category 2 and 3 microplate readers use gradient heating as well. SoftMax Pro Software is an industry-leading acquisition and analysis software with over 160 ready-to-run protocols, powerful data analysis, and a range of data output options. Molecular Devices. SPECTRAmax PLUS 384 Microplate Spectrophotometer Operator’s Manual vii Conventions Used in this Manual The names of keys that appear on the SPECTRAmax PLUS 384 control panel are shown in boxed Helvetica type. SpectraMax ® M5 Multi-Mode Microplate Reader Transcreener ® ADP. The SpectraMax® Paradigm® Multi-Mode Microplate Reader measures absorbance, fluorescence, time-resolved fluorescence (including HTRF), fluorescence polarization, AlphaScreen®, AlphaLISA®, and luminescence assays for up to 1536-well plates. for ValitaTiter Plus standards read on Molecular Devices readers. 4 0. SpectraMax® ABS. Trilevel commercial control reagents (StanbioThe SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual- monochromator microplate reader platform offering a wide range of. 1 & 7. AquaMax® 2000. Five. This. The SpectraMax® ABS Microplate Reader bridges the gap between the affordability of filter-based readers and the . By using PathCheck, SpectraMax readers can automatically normalize sample absorbance values to a 1-cm pathlength and calculate the concentrations. An assay plate containing a 15-point standard curve was read on the ® M5 Microplate reader. metrically at 540 nm using a microplate reader (SpectraMax 384 Plus, Molecular Devices Corp. The Gemini XPS Reader can be integrated with Molecular Devices’ StakMax® Microplate Stacker in a matter of minutes and begin reading microplates with seven mouse clicks. SpectraMax M2, M2e; SpectraMax M3, M4, M5, M5e; FilterMax™ Multi-Mode Detection Platform. IMAP phosphodiesterase assays on SpectraMax Multi-Mode Microplate Readers. After that, the cells were resuspended and diluted to a suitable density using DCFH-DA working solution, transferred to an opaque 96-well plate and incubated for 30 min. 2. Keywords. Table 3 shows sample cell proliferation assay results for CHO-K1 cells plated according to the scheme in Table 1 and allowed to proliferate in culture for two days. Standard features include a. B: Selection of Excitation Wavelength. Water Constant and Cuvette Reference options are available on SpectraMax microplate readers with a cuvette port. SpectraMax® iD5. e.